By Vedpal S. Malik, Erik P. Lillehoj
The applicability of immunotechniques to a large choice of study difficulties in lots of components of biology and chemistry has extended dramatically over the past 20 years ever because the advent of monoclonal antibodies and complex immunosorbent ideas. Exquisitely particular antibody molecules supply technique of separation, quantitative and qualitative research, and localization helpful to someone doing organic or biochemical research.
This useful consultant to immunotechniques is principally designed to be simply understood via individuals with little functional adventure utilizing antibodies. It truly provides precise, easy-to-follow, step by step equipment for the generally used ideas that take advantage of the original houses of antibodies and should support researchers use antibodies to their greatest virtue.
* specific, easy-to-follow, step by step protocols
* handy, easy-to-use format
* huge sensible information
* crucial history information
* worthwhile tricks
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Extra resources for Antibody Techniques
1989). Primary antibody-forming cells and secondary B cells are generated from separate precursor cell subpopulations. Cell 59, 1049-1059. Little, J. , and Eisen, H. N. (1969). Specificity of the immune response to the 2,4dinitrophenyl and 2,4,6-trinitrophenyl groups. /. Exp. Med. 129, 2 4 7 - 2 6 5 . , Trucy, J . , Dunn, D. , Fitch, F. , and Malissen, B. (1988). A T cell clone expresses two T cell receptor a genes but uses one a/3 heterodimer for allorecognition and self MHC-restricted antigen recognition.
5, 2770-2776. Nossal, G. J. V. (1994). Negative selection of lymphocytes. Cell 76, 2 2 9 - 2 3 9 . Oettinger, M. , Schatz, D. , and Baltimore, D. (1990). RAG-1 and RAG2, adjacent genes that synergistically activate V(D)J recombination. Science 248, 1517-1523. Parker, D. C. (1993). T cell-dependent B cell activation. Annu. Rev. Immunol. 33, 3 3 1 - 3 6 0 . Paul, W. E . , and Seder, R. A. (1994). Lymphocyte responses and cytokines. Cell 7 6 , 2 4 1 - 2 5 1 . , and Lanzavecchia, A. (1993). Expression of two T cell receptor a chains: Dual receptor T cells.
And Ledbetter, ). A. (1991a). Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin-2 mRNA accumulation. /. Exp. Med. 173, 7 2 1 - 7 3 0 . Linsley, P. , Grosmaire, L. , Damle, N. , and Ledbetter, J. A. (1991b). CTLA-4 is a second receptor for the B cell activation antigen B7. /. Exp. Med. 174, 5 6 1 - 5 6 9 . Linton, P. J . , Decker, D. J . , and Klinman, N. R. (1989). Primary antibody-forming cells and secondary B cells are generated from separate precursor cell subpopulations.