Advances in Topical Antifungal Therapy by R. J. Hay (auth.), R. J. Hay MD, MRCP, MRCPath. (eds.)

By R. J. Hay (auth.), R. J. Hay MD, MRCP, MRCPath. (eds.)

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Extra resources for Advances in Topical Antifungal Therapy

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Material such as textile fibers or cellulose fibers appear intensely white and, as more visible light is emitted than received, the fabric achieves a brilliant quality. Thus whiteners cause fabrics to glow in ultraviolet light. Fungal cell walls contain chitin (N-acetyl monomer). There is a similarity in the constitution form between chitin and cellulose which may explain why the FWA-stained fungal elements fluoresce when examined under the fluorescence microscope. We like to stress the ease in reading samples using this method due to the contrast between the black background and the brilliant light green fungal elements.

Plempel M, Berg D (1984) Reduction of the in vivo virulence of Candida albicans by pretreatment with subinhibitory azole concentration in vivo. Dermatologica 169, suppl1: 11-18 9. Plempel M, Regel E (1982) Antimycotic properties of the topical azole bifonazole in vivo and in vitro. In: Urabe H, Zaias N, Stettendorf S: International Antifungal Symposium: Bifonazole. Excerpta Medica, Amsterdam, 29-36 10. Reinel D (1985) Lokaltherapie von Dermatomykosen. Z Allgem Med 23: 835-838 31 Differential Staining of Fungi in Clinical Specimens Using Fluorescent Whitening Agent L.

Methods For the sampling from dry skin lesions the transparent ultrathin Scotch Tape 850 (3 M, Los Angeles, California) was used. The skin surface was sampled by placing the tape over an area of the skin and flattening it with a sterile spatula to get close contact. After stripping, the tape was laid, adhesive side down, on a sterile glass slide covered with a 30% KOH solution. Next, the slide was gently heated until boiling by passing through a flame several times. 1 ml of a 23% watery solution of FWA.

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