Biological Sciences

Advances in Giardia Research by Peter Malcolm Wallis, Brian R. Hammond

By Peter Malcolm Wallis, Brian R. Hammond

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40:821­832.  Biophysical and Biochemical Cytology 9:409­413.  2:187­196.  74:429­433.  13:16­18.  Gastroenterology 71:57­61.  53:704­707.  J.  73:723­729.  Gastroenterology, In press. 9­21.  lamblia trophozoites.  These observations suggest that direct cell to parasite contact may play an important role in damaging the host cells.  One reported the significance of bile salt deconjugation by the parasite (12) and the other the cytopathogenic effects of Giardia on cell monolayers, in vitro (11).

Sections were cut on a LKB Huxley * Corresponding author. Page 30 ultramicrotome, stained with uranyl acetate and lead citrate, then examined with a JEOL 100 CX electron microscope.  After one day, examination of the cultures by Figures 1 and 2. Differential interference contrast (DIC) micrographs of a Giardia culture three days after induction of encystation with bile.  In Figure 2, the viable cysts possess a well­defined cyst wall (CW) and peritrophic space (PS).  Bar equals 5 microns. Figures 3 and 4.

After one day, examination of the cultures by Figures 1 and 2. Differential interference contrast (DIC) micrographs of a Giardia culture three days after induction of encystation with bile.  In Figure 2, the viable cysts possess a well­defined cyst wall (CW) and peritrophic space (PS).  Bar equals 5 microns. Figures 3 and 4.  The cyst with nonviable morphology (N) seen in Figure 3 was stained with the fluorogenic dye, PI, as shown in Figure 4, and fluoresced orange. Bar equals 5 microns for Figures 3 and 4.

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